Abstract
Matrix metalloproteinases (MMPs) are a family of proteinases that play an important role in cancer as well as in numerous diseases. In this article, we describe the labeling of a phage display selected cyclic decapeptide containing the HWGF (histidine-tryptophane-glycine-phenylalanine) sequence to target MMP-2 and MMP-9. To evaluate the ability of this labeled peptide to monitor non invasively MMP-2 and MMP-9 activity, in vitro studies, biodistribution, competition studies and plasma metabolites analyses in Lewis Lung cancer tumor bearing mice were performed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biomarkers, Tumor / metabolism*
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Drug Delivery Systems / methods
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Enzyme Activation
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Female
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Gelatinases / metabolism
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Iodine Radioisotopes / chemistry
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Iodine Radioisotopes / pharmacokinetics
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Isotope Labeling / methods
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Lung Neoplasms / diagnostic imaging*
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Lung Neoplasms / metabolism*
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Matrix Metalloproteinase 2 / metabolism*
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Matrix Metalloproteinase 9 / metabolism*
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Metabolic Clearance Rate
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Mice
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Mice, Inbred BALB C
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Organ Specificity
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Peptides, Cyclic / chemical synthesis
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Peptides, Cyclic / pharmacokinetics*
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Radionuclide Imaging
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Radiopharmaceuticals / pharmacokinetics
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Tissue Distribution
Substances
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Biomarkers, Tumor
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Iodine Radioisotopes
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Peptides, Cyclic
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Radiopharmaceuticals
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Gelatinases
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9