Abstract
The pac gene, encoding the penicillin G acylase from Escherichia coli W, is regulated by the PaaX repressor of the phenylacetate catabolic pathway. pac expression depends on the synthesis of phenylacetyl-coenzyme A. PaaX and the cyclic AMP receptor protein (CRP) bind in vitro to the Ppac promoter region. A palindromic sequence proposed as the PaaX operator is located upstream of the -35 box overlapping a CRP binding site, an unusual position that suggests a novel regulatory mechanism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyl Coenzyme A / genetics
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Acetyl Coenzyme A / metabolism*
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Amino Acid Sequence
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Base Sequence
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Binding Sites
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Gene Expression Regulation, Bacterial*
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Molecular Sequence Data
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Penicillin Amidase / genetics
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Penicillin Amidase / metabolism*
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Phenylacetates / metabolism*
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
Substances
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Escherichia coli Proteins
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Phenylacetates
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Repressor Proteins
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Acetyl Coenzyme A
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phenylacetyl-coenzyme A
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Penicillin Amidase