Real-time polymerase chain reaction for detecting SARS coronavirus, Beijing, 2003

Emerg Infect Dis. 2004 Feb;10(2):300-3. doi: 10.3201/eid1002.030799.

Abstract

During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3' end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Viral / blood
  • Base Sequence
  • China / epidemiology
  • DNA Primers / genetics
  • DNA, Viral / genetics
  • Disease Outbreaks
  • Feces / virology
  • Humans
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / standards
  • RNA, Viral / blood
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Severe Acute Respiratory Syndrome / epidemiology
  • Severe Acute Respiratory Syndrome / immunology
  • Severe Acute Respiratory Syndrome / virology
  • Severe acute respiratory syndrome-related coronavirus / genetics*
  • Severe acute respiratory syndrome-related coronavirus / immunology
  • Severe acute respiratory syndrome-related coronavirus / isolation & purification*
  • Time Factors

Substances

  • Antibodies, Viral
  • DNA Primers
  • DNA, Viral
  • RNA, Viral