In this paper, the effect of saccharose on the association of phenol derivatives on both the porous graphitic carbon (PGC) surface and the C18 stationary phase and for two methanol fractions (v/v) in the mobile phase is described. A novel approach based on an extended Langmuir distribution isotherms was used. The results demonstrated that: (i) the saccharose can be adsorbed on the PGC surface; (ii) the phenol derivatives can be associated with saccharose adsorbed on the PGC surface; and (iii) the saccharose do not interact with the C18 stationary phase. This was confirmed by the thermodynamic data and the Wyman equation parameters.