Quantitative autoradiography of dopamine receptors in the brains of micro-opioid receptor knockout mice

Neurosci Lett. 2004 Feb 19;356(3):220-4. doi: 10.1016/j.neulet.2003.11.058.

Abstract

Given the existence of functional interactions between opioidergic and dopaminergic systems, we have analyzed by quantitative autoradiography the possible long-term adaptive changes in the expression of D(1)- and D(2)-like dopamine receptors in the brains of mice lacking the micro-opioid receptor gene. An overall significant increase in D(1) and D(2) receptors (7.4 and 12.6%, respectively) across all cerebral regions examined was obtained in mutant mice relative to wild-type mice. However, region by region comparisons failed to reach significance in any individual brain area. These results indicate that only moderate changes in D(1)- and D(2)-like dopamine receptors densities occur in the brains of micro-opioid receptor knockout mice.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Autoradiography / methods
  • Benzazepines / pharmacokinetics
  • Brain / anatomy & histology
  • Brain / metabolism*
  • Dopamine Antagonists / pharmacokinetics
  • Mice
  • Mice, Knockout
  • Raclopride / pharmacokinetics
  • Receptors, Dopamine / analysis*
  • Receptors, Dopamine / classification
  • Receptors, Opioid, mu / deficiency*
  • Receptors, Opioid, mu / genetics
  • Receptors, Opioid, mu / metabolism
  • Tritium / pharmacokinetics

Substances

  • Benzazepines
  • Dopamine Antagonists
  • Receptors, Dopamine
  • Receptors, Opioid, mu
  • Tritium
  • Raclopride