Automated sequencing and mapping of cosmid DNA with fluorescently-labeled dideoxynucleotide terminators

Biotechniques. 1992 Jul;13(1):46-7.

Abstract

We have established a method for directly sequencing cosmid DNA on an automated DNA sequencer. The major advantage of this method is that only small amounts of cosmid template DNA are needed for the sequencing reactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence*
  • Cosmids / genetics*
  • DNA
  • Genetic Techniques
  • Molecular Sequence Data
  • Nucleotides
  • Restriction Mapping
  • Templates, Genetic

Substances

  • Nucleotides
  • DNA