Abstract
RAV1 is a novel DNA-binding protein with two distinct DNA-binding domains unique in higher plants, but its role in plant growth and development remains unknown. Using cDNA array, we found that transcription of RAV1 is down-regulated by epibrassinolide (epiBL) in Arabidopsis suspension cells. RNA gel blot analysis revealed that epiBL-regulated RAV1 transcription involves neither protein phosphorylation/dephosphorylation nor newly synthesized protein, and does not require the functional BRI1, suggesting that this regulation might be through a new BR signaling pathway. Overexpressing RAV1 in Arabidopsis results in a retardation of lateral root and rosette leaf development, and the underexpression causes an earlier flowering phenotype, implying that RAV1 may function as a negative regulatory component of growth and development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2,4-Dichlorophenoxyacetic Acid / pharmacology
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Arabidopsis / genetics
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Arabidopsis / growth & development*
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Arabidopsis / physiology
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Arabidopsis Proteins / physiology*
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Blotting, Northern
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Blotting, Southern
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Blotting, Western
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Brassinosteroids
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Cholestanols / pharmacology*
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Cycloheximide / pharmacology
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DNA, Antisense / genetics
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology*
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Down-Regulation / drug effects
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Flowers / growth & development
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Gene Dosage
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Gene Expression Regulation, Developmental / drug effects
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Gene Expression Regulation, Plant / drug effects
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Models, Biological
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Mutation / genetics
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Okadaic Acid / pharmacology
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Oligonucleotide Array Sequence Analysis
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Plant Growth Regulators / pharmacology
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Plant Leaves / growth & development
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Plant Roots / growth & development
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Plants, Genetically Modified
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Protein Kinases / metabolism
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Rhizobium / genetics
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Signal Transduction / drug effects
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Staurosporine / pharmacology
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Steroids, Heterocyclic / pharmacology*
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Transformation, Genetic / genetics
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Zeatin / pharmacology
Substances
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Arabidopsis Proteins
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Brassinosteroids
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Cholestanols
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DNA, Antisense
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DNA-Binding Proteins
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Plant Growth Regulators
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RAV1 protein, Arabidopsis
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Steroids, Heterocyclic
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Okadaic Acid
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2,4-Dichlorophenoxyacetic Acid
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Zeatin
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Cycloheximide
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Protein Kinases
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BRI1 protein, Arabidopsis
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Staurosporine
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brassinolide