Multiple trypsin inhibitors from Momordica cochinchinensis seeds, the Chinese drug mubiezhi

Ricardo C H Wong; W P Fong; T B Ng

doi:10.1016/j.peptides.2004.01.002

Multiple trypsin inhibitors from Momordica cochinchinensis seeds, the Chinese drug mubiezhi

Peptides. 2004 Feb;25(2):163-9. doi: 10.1016/j.peptides.2004.01.002.

Authors

Ricardo C H Wong¹, W P Fong, T B Ng

Affiliation

¹ Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

PMID: 15062996
DOI: 10.1016/j.peptides.2004.01.002

Abstract

Five trypsin inhibitors, with N-terminal sequences demonstrating homology to each other and exhibiting a molecular weight of 5100, 4800, 4400, 4100, and 3900, respectively, were isolated from Momordica cochinchinensis seeds with a protocol involving acid extraction, ion exchange chromatography on SP-Sepharose chromatography, and RP-HPLC on a C18 column. Specific inhibitory activity against trypsin was demonstrated by the trypsin isoinhibitors with Ki values ranging from 5.3 x 10(-8) to 1.8 x 10(-6) M. None of the isoinhibitors could be cleaved by trypsin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Chromatography, High Pressure Liquid
Molecular Sequence Data
Momordica / chemistry*
Plant Proteins / chemistry*
Plants, Medicinal / chemistry*
Seeds / chemistry*
Trypsin Inhibitors / chemistry*

Substances

Plant Proteins
Trypsin Inhibitors