Abstract
Bacillus subtilis spores were killed by CuCl(2)-ascorbic acid, chloride ions were essential for killing of spores, and spores with defective coats were killed more rapidly. CuCl(2)-ascorbic acid did not damage spore DNA, and spores killed by this reagent initiated germination. However, spores killed by CuCl(2)-ascorbic acid may have damage to their inner membrane.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Ascorbic Acid / pharmacology
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Bacillus subtilis / drug effects*
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Copper / analysis
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DNA Damage
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DNA, Bacterial / drug effects
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Hydrogen Peroxide / chemistry
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Hydrogen Peroxide / pharmacology*
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Iron / chemistry
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Iron / pharmacology*
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Spores, Bacterial / drug effects
Substances
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DNA, Bacterial
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Fenton's reagent
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Copper
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Hydrogen Peroxide
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Iron
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Ascorbic Acid
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cupric chloride