Abstract
Single-stranded conformation polymorphism (SSCP) analysis and heteroduplex mobility assays (HMAs) were used to identify and genotype enteric adenoviruses (EAd). The results were compared to those of restriction endonuclease assays, species-specific PCRs, and direct nucleotide sequence analyses. Of the 31 stool samples tested, 15 isolates were identified as EAd and 7 were identified as nonenteric Ad by all methods. An agreement of 100% was found between the SSCP and HMA results.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenovirus Infections, Human / virology
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Adenoviruses, Human / classification*
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Adenoviruses, Human / genetics
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Adenoviruses, Human / isolation & purification
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Brazil
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Child, Preschool
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DNA, Viral / analysis
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Diarrhea / virology*
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Feces / virology
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Genotype
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Heteroduplex Analysis / methods*
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Humans
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Infant
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Infant, Newborn
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Molecular Sequence Data
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Polymerase Chain Reaction
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Polymorphism, Single-Stranded Conformational*
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Restriction Mapping
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Sequence Analysis, DNA
Associated data
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GENBANK/AY375456
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GENBANK/AY375457
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GENBANK/AY375458
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GENBANK/AY375459
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GENBANK/AY375460
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GENBANK/AY375461
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GENBANK/AY375462