Abstract
A newly identified cell-cycle master regulator protein, GcrA, together with the CtrA master regulator, are key components of a genetic circuit that drives cell-cycle progression and asymmetric polar morphogenesis in Caulobacter crescentus. The circuit drives out-of-phase temporal and spatial oscillation of GcrA and CtrA concentrations, producing time- and space-dependent transcriptional regulation of modular functions that implement cell-cycle processes. The CtrA/GcrA regulatory circuit controls expression of polar differentiation factors and the timing of DNA replication. CtrA functions as a silencer of the replication origin and GcrA as an activator of components of the replisome and the segregation machinery.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Caulobacter crescentus / cytology*
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Caulobacter crescentus / genetics*
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Caulobacter crescentus / growth & development
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Caulobacter crescentus / metabolism
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Cell Cycle / genetics*
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DNA Replication
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Expression Profiling
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Gene Expression Regulation, Bacterial*
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Gene Silencing
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Genes, Bacterial
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Genes, Essential
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Oligonucleotide Array Sequence Analysis
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Promoter Regions, Genetic
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Replication Origin
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic
Substances
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Bacterial Proteins
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CtrA protein, Caulobacter
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DNA-Binding Proteins
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Transcription Factors