Abstract
Tau is a microtubule-associated protein whose transcript undergoes complex-regulated splicing in the mammalian nervous system. Exon 10 of the gene is an alternatively spliced cassette, which is adult-specific and codes for a microtubule-binding domain. Mutations that affect splicing of exon 10 have been shown to cause frontotemporal dementia with parkinsonism (FTDP). Using tau exon 10 as a bait in a yeast three-hybrid screen, we discovered that it interacts with hnRNPE2. Cotransfection assays show that hnRNPE2 isoforms moderately activate the splicing of exon 10.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Alternative Splicing*
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Animals
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Base Sequence
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Brain / embryology
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Brain / metabolism
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COS Cells
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Cerebellum / metabolism
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Cerebral Cortex / metabolism
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Chlorocebus aethiops
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Exons / genetics*
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Gene Expression
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Gene Expression Regulation, Developmental
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Heterogeneous-Nuclear Ribonucleoproteins / genetics
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Heterogeneous-Nuclear Ribonucleoproteins / metabolism*
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Humans
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Molecular Sequence Data
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Protein Binding
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Two-Hybrid System Techniques
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Yeasts / genetics
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tau Proteins / genetics*
Substances
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DNA-Binding Proteins
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Heterogeneous-Nuclear Ribonucleoproteins
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tau Proteins