Abstract
Connective-tissue growth factor (CTGF) is a member of the CCN family of secreted proteins. CCN family members contain four characteristic domains and exhibit multiple activities: they associate with the extracellular matrix, they can mediate cell adhesion, cell migration and chemotaxis, and they can modulate the activities of peptide growth factors. Many of the effects of CTGF are thought to be mediated by binding to integrins, whereas others may be because of its recently identified ability to interact with BMP4 and TGF beta. We demonstrate, using Xenopus embryos, that CTGF also regulates signalling through the Wnt pathway, in accord with its ability to bind to the Wnt co-receptor LDL receptor-related protein 6 (LRP6). This interaction is likely to occur through the C-terminal (CT) domain of CTGF, which is distinct from the BMP- and TGF beta-interacting domain. Our results define new activities of CTGF and add to the variety of routes through which cells regulate growth factor activity in development, disease and tissue homeostasis.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Connective Tissue Growth Factor
-
Embryonic Induction
-
Gene Expression Regulation, Developmental
-
Genes, Reporter
-
Immediate-Early Proteins / genetics
-
Immediate-Early Proteins / metabolism*
-
Intercellular Signaling Peptides and Proteins / genetics
-
Intercellular Signaling Peptides and Proteins / metabolism*
-
Low Density Lipoprotein Receptor-Related Protein-6
-
Macromolecular Substances
-
Molecular Sequence Data
-
Morphogenesis / physiology
-
Oligonucleotides, Antisense / genetics
-
Oligonucleotides, Antisense / metabolism
-
Proto-Oncogene Proteins / genetics
-
Proto-Oncogene Proteins / metabolism*
-
Receptors, LDL / genetics
-
Receptors, LDL / metabolism*
-
Sequence Alignment
-
Signal Transduction / physiology*
-
Wnt Proteins
-
Xenopus Proteins / genetics
-
Xenopus Proteins / metabolism
-
Xenopus laevis / anatomy & histology
-
Xenopus laevis / embryology
-
Zebrafish Proteins*
Substances
-
Immediate-Early Proteins
-
Intercellular Signaling Peptides and Proteins
-
Low Density Lipoprotein Receptor-Related Protein-6
-
Macromolecular Substances
-
Oligonucleotides, Antisense
-
Proto-Oncogene Proteins
-
Receptors, LDL
-
Wnt Proteins
-
Xenopus Proteins
-
Zebrafish Proteins
-
Connective Tissue Growth Factor