Abstract
The differences in gene expression pattern of visceral white adipose tissue between control and high-fat-fed rats were compared using the mRNA differential display methodology. The results, confirmed by Northern blot, showed eight genes upregulated: adiponectin, fibrillin-1, transferrin, Y-box binding protein-1, IgE receptor beta chain (FcRIbeta), alpha-1 haemoglobin, and ribosomal proteins S10 and L7 and four genes downregulated: caveolin-2, lactate dehydrogenase-A, mitochondrial 16S rRNA, and mitochondrial cytochrome oxidase subunit I/serine tRNA. Two of these genes have been already related to obesity (adiponectin and caveolin-2) while the others are known to participate in metabolic, signalling or transcription regulation pathways that can be relevant in energy (lipid and/or carbohydrate) metabolism.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue / drug effects*
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Adipose Tissue / metabolism*
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Adipose Tissue / physiology
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Animals
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Caveolin 1
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Caveolin 2
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Caveolins / biosynthesis
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Dietary Fats / administration & dosage*
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Down-Regulation / drug effects
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Female
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Fibrillin-1
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Fibrillins
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Gene Expression Profiling
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Gene Expression Regulation / drug effects*
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L-Lactate Dehydrogenase / biosynthesis
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Microfilament Proteins / biosynthesis
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Protein Biosynthesis
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Proteins / genetics
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Rats
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Rats, Wistar
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Up-Regulation / drug effects
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Weight Gain
Substances
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Cav1 protein, rat
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Caveolin 1
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Caveolin 2
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Caveolins
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Dietary Fats
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Fbn1 protein, rat
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Fibrillin-1
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Fibrillins
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Microfilament Proteins
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Proteins
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RNA, Messenger
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L-Lactate Dehydrogenase