Studying vaccinia virus RNA processing in vitro

Methods Mol Biol. 2004:269:151-68. doi: 10.1385/1-59259-789-0:151.

Abstract

The study and use of vaccinia virus-derived RNA-modifying proteins has made a significant contribution to molecular biology. Here, the purification and assay of two such proteins, comprising the vaccinia poly(A) polymerase/cap-specific mRNA 2'-O-methyltransferase, is described.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Cell Line
  • In Vitro Techniques
  • Nucleotidyltransferases / genetics
  • Nucleotidyltransferases / metabolism*
  • RNA Processing, Post-Transcriptional*
  • RNA, Messenger / metabolism
  • RNA, Viral / metabolism*
  • Vaccinia virus / enzymology
  • Vaccinia virus / genetics
  • Vaccinia virus / metabolism*

Substances

  • RNA, Messenger
  • RNA, Viral
  • Nucleotidyltransferases