Abstract
Platelet-derived growth factor and phorbol ester cause an increase in vascular endothelial growth factor (VEGF) mRNA expression in control NIH 3T3 fibroblasts and NIH 3T3 fibroblasts overexpressing human protein kinase C (PKC) alpha. In the case of phorbol ester-induced VEGF expression, the VEGF mRNA levels were significantly higher in cells overexpressing human PKC alpha as compared to control cells. In cells stimulated with platelet-derived growth factor or phorbol ester, induction of expression was lost after down-regulation of PKC. This indicates that PKC is involved in the signal transduction leading to VEGF expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Endothelial Growth Factors / genetics*
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Gene Expression / drug effects
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Lymphokines / genetics*
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Mice
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Platelet-Derived Growth Factor / pharmacology*
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Protein Kinase C / physiology*
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RNA, Messenger / genetics
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Tetradecanoylphorbol Acetate / pharmacology
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Time Factors
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Transcription, Genetic / drug effects
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Transfection
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
Substances
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Endothelial Growth Factors
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Lymphokines
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Platelet-Derived Growth Factor
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RNA, Messenger
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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Protein Kinase C
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Tetradecanoylphorbol Acetate