Evaluation of antiviral activity against human herpesvirus 8 (HHV-8) and Epstein-Barr virus (EBV) by a quantitative real-time PCR assay

Claudia Friedrichs; Johan Neyts; Gabor Gaspar; Erik De Clercq; Peter Wutzler

doi:10.1016/j.antiviral.2003.12.005

Evaluation of antiviral activity against human herpesvirus 8 (HHV-8) and Epstein-Barr virus (EBV) by a quantitative real-time PCR assay

Antiviral Res. 2004 Jun;62(3):121-3. doi: 10.1016/j.antiviral.2003.12.005.

Authors

Claudia Friedrichs¹, Johan Neyts, Gabor Gaspar, Erik De Clercq, Peter Wutzler

Affiliation

¹ Institute of Virology and Antiviral Therapy, Friedrich-Schiller University of Jena, Jena, Germany.

PMID: 15130535
DOI: 10.1016/j.antiviral.2003.12.005

Abstract

A real-time quantitative PCR was developed to assess antiviral activity of molecules against human herpesvirus 8 (HHV-8) and the Epstein-Barr virus (EBV). The antiviral activity of the reference molecules acyclovir, ganciclovir, cidofovir, adefovir and brivudin, as assessed by this methodology, proved very similar to the activity as determined by a DNA-DNA hybridisation method.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acyclovir / pharmacology
Antiviral Agents / blood
Antiviral Agents / pharmacology*
Cell Line
Cidofovir
Cytosine / analogs & derivatives*
Cytosine / pharmacology
Ganciclovir / pharmacology
Herpesvirus 4, Human / drug effects*
Herpesvirus 4, Human / genetics
Herpesvirus 4, Human / immunology
Herpesvirus 8, Human / drug effects*
Herpesvirus 8, Human / genetics
Herpesvirus 8, Human / immunology
Humans
Microbial Sensitivity Tests
Organophosphonates*
Organophosphorus Compounds / pharmacology
Polymerase Chain Reaction

Substances

Antiviral Agents
Organophosphonates
Organophosphorus Compounds
Cytosine
Cidofovir
Ganciclovir
Acyclovir