Development and evaluation of a new commercial test allowing the simultaneous detection of noroviruses and sapoviruses by reverse transcription-PCR and microplate hybridization

F Bon; H Giraudon; C Sancey; C Barranger; M Joannes; P Pothier; E Kohli

doi:10.1128/JCM.42.5.2218-2220.2004

Development and evaluation of a new commercial test allowing the simultaneous detection of noroviruses and sapoviruses by reverse transcription-PCR and microplate hybridization

J Clin Microbiol. 2004 May;42(5):2218-20. doi: 10.1128/JCM.42.5.2218-2220.2004.

Authors

F Bon¹, H Giraudon, C Sancey, C Barranger, M Joannes, P Pothier, E Kohli

Affiliation

¹ Laboratoire de Virologie, Reference Laboratory for Enteric Viruses, Centre Hospitalier Universitaire, and Microbiologie Médicale et Moléculaire, UFRS Médecine et Pharmacie, Dijon, France.

Abstract

This work describes the design and initial evaluation of a commercial test allowing the detection of noroviruses and sapoviruses by reverse transcription-PCR (RT-PCR) in a single tube followed by microplate hybridization, as well as the detection of PCR inhibitors. The test was shown to be broadly reactive (except for Melksham-like strains), sensitive, and specific and thus should be useful for calicivirus detection in clinical practice.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Caliciviridae Infections / epidemiology
Caliciviridae Infections / virology
Disease Outbreaks
Gastroenteritis / epidemiology
Gastroenteritis / virology
Humans
Norovirus / classification
Norovirus / genetics*
Norovirus / isolation & purification*
Nucleic Acid Hybridization / methods
Reverse Transcriptase Polymerase Chain Reaction / methods*
Reverse Transcriptase Polymerase Chain Reaction / statistics & numerical data
Sapovirus / classification
Sapovirus / genetics*
Sapovirus / isolation & purification*
Sensitivity and Specificity
Virology / methods*
Virology / statistics & numerical data