[Preparation and identification of phage-displayed ICAM-1-mimic peptide]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2003 Jan;19(1):77-9.
[Article in Chinese]

Abstract

Aim: To obtain bioactive ICAM-1 mimetic peptide.

Methods: Phages displaying P1 and P2 were prepared by phage amplication and PEG precipitation. The binding between phage-displayed peptides and anti-ICAM-1 mAb 15.2 was evaluated by sandwich ELISA and competitive ELISA. Bioactivities of P1 and P2 was detected by immunohistochemical staining.

Results: Phage-displayed peptides P1 and P2 could specifically bind to mAb 15.2, and the binding could be competitively inhibited by ICAM-1. Immunohistochemical staining showed that P1 and P2 could mimic the binding of ICAM-1 to its receptor LFA-1.

Conclusion: Phage-displayed peptides P1 and P2 are bioactive just as native ICAM-1.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Bacteriophage P1 / genetics
  • Bacteriophage P1 / metabolism
  • Bacteriophage P2 / genetics
  • Bacteriophage P2 / metabolism
  • Binding Sites
  • Intercellular Adhesion Molecule-1 / genetics
  • Intercellular Adhesion Molecule-1 / immunology
  • Intercellular Adhesion Molecule-1 / metabolism*
  • Lymphocyte Function-Associated Antigen-1 / metabolism*
  • Peptide Library*
  • Peptides / genetics
  • Peptides / metabolism*
  • Protein Binding

Substances

  • Antibodies, Monoclonal
  • Lymphocyte Function-Associated Antigen-1
  • Peptide Library
  • Peptides
  • Intercellular Adhesion Molecule-1