Abstract
Ischemia-reperfusion injury, a clinical problem during cardiac surgery, involves worsened adenosine trisphosphate (ATP) generation and damage to the heart. We studied carbon monoxide (CO) pretreatment, proven valuable in rodents but not previously tested in large animals, for its effects on pig hearts subjected to cardiopulmonary bypass with cardioplegic arrest. Hearts of CO-treated pigs showed significantly higher ATP and phosphocreatine levels, less interstitial edema, and apoptosis of cardiomyocytes and required fewer defibrillations after bypass. We conclude that treatment with CO improves the energy status, prevents edema formation and apoptosis, and facilitates recovery in a clinically relevant model of cardiopulmonary bypass surgery.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Diphosphate / metabolism
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Adenosine Monophosphate / metabolism
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Adenosine Triphosphate / metabolism
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Animals
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Apoptosis / drug effects
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Carbon Monoxide / pharmacology*
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Carbon Monoxide / therapeutic use
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Cardiopulmonary Bypass* / adverse effects
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Cardiotonic Agents / pharmacology*
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Cardiotonic Agents / therapeutic use
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Edema / prevention & control
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Electric Countershock
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Energy Metabolism / drug effects
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Female
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Guanosine Triphosphate / metabolism
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Heart / drug effects*
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Myocardial Ischemia / drug therapy*
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Myocardial Ischemia / metabolism
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Myocardial Ischemia / therapy
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Myocardial Reperfusion Injury / drug therapy*
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Myocardial Reperfusion Injury / metabolism
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Myocytes, Cardiac / drug effects
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Myocytes, Cardiac / metabolism
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Myocytes, Cardiac / pathology
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NAD / metabolism
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NADP / metabolism
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Oxidation-Reduction
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Sus scrofa
Substances
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Cardiotonic Agents
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NAD
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Adenosine Monophosphate
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NADP
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Adenosine Diphosphate
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Carbon Monoxide
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Guanosine Triphosphate
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Adenosine Triphosphate