Abstract
The intermediate filament protein nestin is expressed in the neural stem cells of the developing central nervous system (CNS). Promoter analysis revealed that the minimal promoter of the mouse nestin gene resides in the region -11 to +183 of the 5'-non-coding and upstream flanking region, and that two adjacent Sp1-binding sites are necessary for promoter activity. Electrophoretic mobility-shift assays (EMSA) and supershift assays showed that Sp1 and Sp3 proteins selectively bind to the upstream Sp1 site. These results demonstrate an important functionality of Sp1 and Sp3 in regulating the expression of the mouse nestin gene.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Brain / embryology
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CHO Cells
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Cell Line
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Cell Line, Tumor
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Cricetinae
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DNA Primers
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DNA, Complementary / metabolism
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DNA-Binding Proteins / metabolism
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Genes, Reporter
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Humans
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Intermediate Filament Proteins / genetics*
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Luciferases / metabolism
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Mice
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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NIH 3T3 Cells
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Nerve Tissue Proteins / genetics*
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Nestin
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Neurons / cytology
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Plasmids / metabolism
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Poly A / chemistry
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Promoter Regions, Genetic*
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RNA / chemistry
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Sp1 Transcription Factor / metabolism
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Sp3 Transcription Factor
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Stem Cells / cytology
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Transcription Factors / metabolism
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Transcription, Genetic
Substances
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DNA Primers
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DNA, Complementary
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DNA-Binding Proteins
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Intermediate Filament Proteins
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NES protein, human
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Nerve Tissue Proteins
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Nes protein, mouse
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Nestin
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SP3 protein, human
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Sp1 Transcription Factor
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Sp3 protein, mouse
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Transcription Factors
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Sp3 Transcription Factor
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Poly A
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RNA
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Luciferases