Abstract
In the lung, caveolin-1 is expressed in both type I alveolar epithelial and endothelial cells where it is hypothesized to modulate molecular signaling activities and progression of tumorigenesis. Developmentally, caveolin-1alpha is expressed in fetal lung endothelial, but not epithelial, cells; in adult lung, both cell types express caveolin-1alpha. To test the hypothesis that caveolin-1 transcription is differentially regulated in type I and endothelial cells, we characterized the proximal promoter of the mouse caveolin-1 gene in lung cell lines to identify factors that control its cell-specific expression. We show that caveolin-1 expression is regulated by an Ets cis-element in a lung epithelial cell line, but not a lung endothelial cell line, and that three ETS family members, ETS-1, PEA3, and ERM, recognize and bind the Ets site in the epithelial cell line. Based on these findings, we have identified the Ets cis-element as a region that accounts for differential transcriptional regulation of caveolin-1 in lung epithelial and endothelial cells.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Motifs
-
Animals
-
Binding Sites
-
Blotting, Western
-
Caveolin 1
-
Caveolins / biosynthesis*
-
Caveolins / genetics*
-
Cell Line
-
Cell Nucleus / metabolism
-
DNA / chemistry
-
DNA Mutational Analysis
-
DNA Primers / pharmacology
-
DNA-Binding Proteins / metabolism
-
Endothelial Cells / metabolism*
-
Enhancer Elements, Genetic
-
Gene Deletion
-
Lung / cytology
-
Mice
-
Mutation
-
Oligonucleotides / chemistry
-
Polymerase Chain Reaction
-
Precipitin Tests
-
Promoter Regions, Genetic
-
Protein Binding
-
Protein Isoforms
-
Proto-Oncogene Protein c-ets-1
-
Proto-Oncogene Proteins / metabolism
-
Proto-Oncogene Proteins c-ets
-
RNA, Messenger / metabolism
-
Reverse Transcriptase Polymerase Chain Reaction
-
Transcription Factors / metabolism
-
Transcription, Genetic*
-
Transfection
Substances
-
Cav1 protein, mouse
-
Caveolin 1
-
Caveolins
-
DNA Primers
-
DNA-Binding Proteins
-
Ets1 protein, mouse
-
Etv5 protein, mouse
-
Oligonucleotides
-
Protein Isoforms
-
Proto-Oncogene Protein c-ets-1
-
Proto-Oncogene Proteins
-
Proto-Oncogene Proteins c-ets
-
RNA, Messenger
-
Transcription Factors
-
transcription factor PEA3
-
DNA