Abstract
Human and animal studies have linked n-6 polyunsaturated fatty acids with mammary carcinogenesis. We investigated the cellular and molecular effects of linoleic acid on the human breast cancer cell line T47D. Linoleic acid had a stimulatory effect on the growth of T47D cells, associated with an increase in the proportion of cells in the S phase of the cell cycle. Microarray, functional group and quantitative PCR analyses indicate that linoleic acid may affect T47D cell growth by modulation of the estrogen receptor (ERalpha), the G13alpha G protein, and p38 MAP kinase gene expression as well genes involved in RNA transcription and cell cycle regulation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cell Division
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Cell Line, Tumor
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Cell Survival
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Dose-Response Relationship, Drug
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Estrogen Receptor alpha
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GTP-Binding Protein alpha Subunits, G12-G13 / metabolism
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Gene Expression Regulation, Neoplastic*
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Humans
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Linoleic Acid / pharmacology*
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Mitogen-Activated Protein Kinases / metabolism
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Oligonucleotide Array Sequence Analysis
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Oligonucleotides / chemistry
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Polymerase Chain Reaction
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RNA / metabolism
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Receptors, Estrogen / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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S Phase
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Transcription, Genetic
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Up-Regulation
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p38 Mitogen-Activated Protein Kinases
Substances
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Estrogen Receptor alpha
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Oligonucleotides
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Receptors, Estrogen
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RNA
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Linoleic Acid
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Mitogen-Activated Protein Kinases
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p38 Mitogen-Activated Protein Kinases
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GTP-Binding Protein alpha Subunits, G12-G13