Abstract
Two particularly well-conserved stretches in the RecA protein sequences were chosen as templates to synthesize degenerate oligonucleotides, which were used in polymerase chain reaction to amplify an internal recA DNA fragment of Lactococcus lactis subsp. lactis ML3. Using this fragment, we recovered and sequenced the entire lactococcal recA gene. The end of an open reading frame present upstream of the recA gene shows strong homology with formamidopyrimidine-DNA-glycosylase, a protein involved in DNA repair.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / genetics
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Base Sequence
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Cloning, Molecular
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DNA Probes
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DNA, Bacterial / genetics
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Escherichia coli / genetics
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Genes, Bacterial*
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Lactococcus lactis / genetics*
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Molecular Sequence Data
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Polymerase Chain Reaction / methods*
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Rec A Recombinases / genetics
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Sequence Homology, Nucleic Acid
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Species Specificity
Substances
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DNA Probes
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DNA, Bacterial
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Rec A Recombinases
Associated data
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GENBANK/D10013
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GENBANK/M55230
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GENBANK/M55231
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GENBANK/M55232
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GENBANK/M55233
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GENBANK/M55234
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GENBANK/M80352
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GENBANK/M80353
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GENBANK/M80354
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GENBANK/M88106