Abstract
Bordetella pertussis secretes an adenylate cyclase toxin (CyaA or ACT) that targets primarily cells expressing the alphaMbeta2 integrin (CD11b/CD18) receptor. This toxin can deliver its N-terminal catalytic AC domain (400 amino acid residues) into the cytosol directly across the cytoplasmic membrane. Various heterologous CD8+, as well as CD4+ T-cell epitopes have been engineered into genetically detoxified CyaA and the resulting toxoids were successfully used as vectors for delivery of inserted epitopes into antigen-presenting cells. Upon processing and presentation, these recombinant CyaAs trigger specific MHC class I and/or class II-restricted T-cell responses both in vitro and in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Adenylate Cyclase Toxin / immunology*
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Antigen Presentation / immunology
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Antigens, Bacterial / immunology*
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Bordetella Infections / immunology*
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Bordetella Infections / prevention & control
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Bordetella pertussis / enzymology
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Bordetella pertussis / immunology*
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CD4-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes
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Dendritic Cells / immunology*
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Epitopes, T-Lymphocyte / immunology
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Histocompatibility Antigens Class I / immunology
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Histocompatibility Antigens Class II / immunology
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Humans
Substances
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Adenylate Cyclase Toxin
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Antigens, Bacterial
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Epitopes, T-Lymphocyte
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Histocompatibility Antigens Class I
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Histocompatibility Antigens Class II