Detection of molecular deletions in the Chinese DMD patients using two amplified dystrophin sequences

Y T Zeng; M J Chen; Z R Ren; Y Huang; X K Qiu; S Z Huang

doi:10.1016/0885-4505(92)90024-s

Detection of molecular deletions in the Chinese DMD patients using two amplified dystrophin sequences

Biochem Med Metab Biol. 1992 Apr;47(2):195-7. doi: 10.1016/0885-4505(92)90024-s.

Authors

Y T Zeng¹, M J Chen, Z R Ren, Y Huang, X K Qiu, S Z Huang

Affiliation

¹ Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, China.

PMID: 1515177
DOI: 10.1016/0885-4505(92)90024-s

Abstract

This Brief Communication reports the detection of molecular deletions in Chinese DMD patients using two new amplified dystrophin DNAs involving the regions of exon 49 and 50. The results show that over 50% of the DMD deletions can be rapidly detected by PCR amplification of these two dystrophin sequences.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
China
Chromosome Deletion*
DNA / blood
DNA / genetics*
DNA / isolation & purification
Dystrophin / genetics*
Exons
Humans
Leukocytes / physiology
Molecular Sequence Data
Muscular Dystrophies / blood
Muscular Dystrophies / genetics*
Oligodeoxyribonucleotides
Oligonucleotide Probes
Polymerase Chain Reaction / methods

Substances

Dystrophin
Oligodeoxyribonucleotides
Oligonucleotide Probes
DNA