IMP-1 and a novel metallo-beta-lactamase, VIM-6, in fluorescent pseudomonads isolated in Singapore

Antimicrob Agents Chemother. 2004 Jun;48(6):2334-6. doi: 10.1128/AAC.48.6.2334-2336.2004.

Abstract

Four carbapenem-resistant Pseudomonas spp. were isolated from patients in Singapore. One Pseudomonas putida isolate contained a bla(IMP-1) identical to that first described in Japan. The sequence of a variant bla(IMP-1) in Pseudomonas fluorescens contained four silent mutations compared with the original sequence. The remaining P. putida isolates contained bla(VIM-6), a novel VIM gene variant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Southern
  • Conjugation, Genetic
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial
  • Isoelectric Focusing
  • Molecular Sequence Data
  • Plasmids / genetics
  • Pseudomonas Infections / microbiology
  • Pseudomonas fluorescens / drug effects
  • Pseudomonas fluorescens / enzymology*
  • Pseudomonas putida / drug effects
  • Pseudomonas putida / enzymology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Singapore
  • beta-Lactamases / metabolism*

Substances

  • DNA, Bacterial
  • beta-lactamase IMP-1
  • beta-Lactamases

Associated data

  • GENBANK/AY165025
  • GENBANK/AY250709
  • GENBANK/AY251052