Expression of protein kinase C isoforms and interleukin-1beta in myofibrillar myopathy

Neurology. 2004 May 25;62(10):1778-82. doi: 10.1212/01.wnl.0000125197.70310.8e.

Abstract

Background: The term myofibrillar myopathy refers to a rare and clinically heterogeneous group of muscle disorders. The pathogenesis of this myopathy is not well understood. The morphologic hallmark is myofibrillar destruction with abnormal expression of numerous proteins, most consistently of desmin.

Methods: The authors investigated eight patients with myofibrillar myopathy belonging to four families. They studied the role of different protein kinase C isoforms and of interleukin-1beta, a cytokine that might activate protein kinase C and, in addition, mediate myofibrillar proteolysis.

Results: Immunohistochemical analysis showed the expression of alpha, eta, and zeta isoforms of protein kinase C and of interleukin-1beta in abnormal muscle fibers. Immunoblots confirmed the immunohistochemical data and revealed the absence of protein kinase C delta and epsilon in muscle fibers from patients and controls.

Conclusions: These data suggest that protein kinase C and interleukin-1beta may play a role in the pathogenesis of myofibrillar myopathy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / deficiency
  • Adult
  • Desmin / metabolism
  • Female
  • Humans
  • Interleukin-1 / analysis*
  • Isoenzymes / analysis
  • Isoenzymes / deficiency
  • Male
  • Middle Aged
  • Muscle Weakness / genetics
  • Muscle Weakness / metabolism
  • Muscle Weakness / pathology
  • Muscle, Skeletal / chemistry
  • Muscle, Skeletal / ultrastructure
  • Muscular Diseases / genetics
  • Muscular Diseases / metabolism*
  • Muscular Diseases / pathology
  • Myofibrils / pathology
  • Oxidative Stress
  • Phosphorylation
  • Protein Kinase C / analysis*
  • Protein Kinase C / deficiency
  • Protein Processing, Post-Translational
  • Respiratory Insufficiency / genetics
  • Respiratory Insufficiency / metabolism
  • Respiratory Insufficiency / pathology

Substances

  • Desmin
  • Interleukin-1
  • Isoenzymes
  • Protein Kinase C
  • Adenosine Triphosphatases