Protein kinase C beta is dispensable for TCR-signaling

Nikolaus Thuille; Thomas Gruber; Günther Böck; Michael Leitges; Gottfried Baier

doi:10.1016/j.molimm.2004.03.007

Protein kinase C beta is dispensable for TCR-signaling

Mol Immunol. 2004 Jun;41(4):385-90. doi: 10.1016/j.molimm.2004.03.007.

Authors

Nikolaus Thuille¹, Thomas Gruber, Günther Böck, Michael Leitges, Gottfried Baier

Affiliation

¹ Department for Medical Biology and Human Genetics, Innsbruck Medical University, Schoepfstrasse 41, A-6020 Innsbruck, Austria.

PMID: 15163535
DOI: 10.1016/j.molimm.2004.03.007

Abstract

PKCbeta has been established to be essential in B cell receptor (BCR) signaling. Additionally, a critical role of PKCbeta in TCR/CD28-stimulated regulation of IL-2 gene transcription but also exocytotic IL-2 secretion was observed in leukemic T cell lines. To now study the physiological function of PKCbeta in primary CD3(+) T cells, we used our established PKCbeta null mice. Unexpectantly, we did not reveal any defect in the development and function of T cells. Proliferative responses as well as IL-2 cytokine secretion of PKCbeta-deficient CD3(+) T cells induced by allogenic MHC, plate-bound anti-CD3 antibodies (with or without anti-CD28 costimulation), or mitogenic stimuli such as phorbol ester and Ca(2+) ionophore were comparable with wild-type controls. Thus, PKCbeta-deficient T cells had similar physiological thresholds for activation in vitro. These findings suggest that PKCbeta plays a redundant role in TCR-induced regulation of IL-2 cytokine production and T cell proliferation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CD28 Antigens / immunology
Cell Division
Genes, Reporter
Humans
Interleukin-2 / genetics
Interleukin-2 / metabolism
Ionomycin / pharmacology
Ionophores / pharmacology
Isoenzymes / genetics
Isoenzymes / physiology
Jurkat Cells
Luciferases / biosynthesis
Luciferases / genetics
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Mice, Knockout
Muromonab-CD3 / pharmacology
Phorbol 12,13-Dibutyrate / pharmacology
Promoter Regions, Genetic / genetics
Protein Kinase C / deficiency
Protein Kinase C / genetics
Protein Kinase C / physiology*
Protein Kinase C-theta
Receptors, Antigen, T-Cell / physiology*
Recombinant Fusion Proteins / physiology
Signal Transduction
T-Lymphocytes / enzymology*
T-Lymphocytes / immunology
Transfection

Substances

CD28 Antigens
Interleukin-2
Ionophores
Isoenzymes
Muromonab-CD3
Receptors, Antigen, T-Cell
Recombinant Fusion Proteins
Phorbol 12,13-Dibutyrate
Ionomycin
Luciferases
PRKCQ protein, human
Prkcq protein, mouse
Protein Kinase C
Protein Kinase C-theta