Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510

Carbohydr Res. 2004 Jun 1;339(8):1475-81. doi: 10.1016/j.carres.2004.03.010.

Abstract

Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / chemistry*
  • Bacteria / enzymology*
  • Chromatography, High Pressure Liquid
  • Glucuronic Acid / chemistry*
  • Hexuronic Acids / chemistry*
  • Magnetic Resonance Spectroscopy
  • Oligosaccharides / chemistry*
  • Oligosaccharides / isolation & purification
  • Oligosaccharides / metabolism*
  • Polysaccharide-Lyases / metabolism*
  • Spectrometry, Mass, Electrospray Ionization

Substances

  • Alginates
  • Hexuronic Acids
  • Oligosaccharides
  • Glucuronic Acid
  • Polysaccharide-Lyases
  • poly(beta-D-mannuronate) lyase