Identification of key immune mediators regulating T helper 1 responses in swine

Harry D Dawson; Atabak R Royaee; Sandra Nishi; Daniel Kuhar; William M Schnitzlein; Federico Zuckermann; Joseph Urban Jr; Joan K Lunney

doi:10.1016/j.vetimm.2004.03.006

Identification of key immune mediators regulating T helper 1 responses in swine

Vet Immunol Immunopathol. 2004 Jul;100(1-2):105-11. doi: 10.1016/j.vetimm.2004.03.006.

Authors

Harry D Dawson¹, Atabak R Royaee, Sandra Nishi, Daniel Kuhar, William M Schnitzlein, Federico Zuckermann, Joseph Urban Jr, Joan K Lunney

Affiliation

¹ Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, USDA, Bldg. 307C, Room 213, BARC-East, Beltsville, MD 20705, USA.

PMID: 15183000
DOI: 10.1016/j.vetimm.2004.03.006

Abstract

This publication describes the cloning of full or partial length sequences for pig TBX21 (T-bet), MYD88, ICSBP1, CD8A (CD8alpha), CD8B (CD8beta), and CD28 cDNAs. Real-time PCR assays have been developed for the relative quantitation of these products as well as previously characterized transcripts that encode exon A-containing CD45, HLX1, IRF1, STAT1 and RPL32. When used for examining temporal immune gene expression in the liver of Toxoplasma gondii infected pigs, the positive regulators of Th1 responses, IRF1, MYD88, and STAT1, were found to be expressed prior to the simultaneous upregulation of interferon gamma (IFNG), HLX1 and TBX21 gene expression. In contrast, in the mesenteric lymph node (MLN), only expression of IRF1 and IFNG was significantly upregulated. Based on their demonstrated utility in establishing an immune response pathway, these PCR assays should be valuable additions to our swine immune toolkit.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Adaptor Proteins, Signal Transducing
Animals
Antigens, Differentiation / genetics
Antigens, Differentiation / immunology
Base Sequence
CD28 Antigens / genetics
CD28 Antigens / immunology
CD8 Antigens / genetics
CD8 Antigens / immunology
Molecular Sequence Data
Myeloid Differentiation Factor 88
RNA, Protozoan / chemistry
RNA, Protozoan / genetics
Receptors, Immunologic / genetics
Receptors, Immunologic / immunology
Repressor Proteins / genetics
Repressor Proteins / immunology
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Sequence Analysis, DNA
Swine
Swine Diseases / immunology
Swine Diseases / parasitology*
T-Box Domain Proteins / genetics
T-Box Domain Proteins / immunology
Th1 Cells / immunology*
Toxoplasma / genetics
Toxoplasma / immunology*
Toxoplasmosis, Animal / immunology*
Toxoplasmosis, Animal / parasitology

Substances

Adaptor Proteins, Signal Transducing
Antigens, Differentiation
CD28 Antigens
CD8 Antigens
CD8alpha antigen
CD8beta antigen
Myeloid Differentiation Factor 88
RNA, Protozoan
Receptors, Immunologic
Repressor Proteins
T-Box Domain Proteins