Abstract
We analyzed here the expression of the prosurvival Bcl-2 homologue A1 in peripheral B cell compartment. We observed that A1 mRNA are highly expressed in peripheral B cells as compared with other anti-apoptotic genes of the Bcl-2 family such as bcl-xl and bcl-2 itself. The expression of A1 is up-regulated in immature B cells at the transition between transitional type 1 (T1) and type 2 (T2) cells, and remained highly expressed in mature (M) B cells. We, therefore, analyzed the effect of B cell antigen receptor (BCR) and BAFF receptor (BAFF-R) engagement on the regulation of A1 in total B220(+) cells but also FACS-sorted immature T1, T2 and M B cells. We demonstrated that only BCR engagement up-regulated the expression of A1 mRNA and protein. These results suggest that A1 may play a key role in antigen-dependent signals that are required for survival and/or proliferation of peripheral B cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis*
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B-Cell Activation Factor Receptor
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B-Lymphocytes / metabolism*
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Gene Expression Regulation*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Minor Histocompatibility Antigens
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Proto-Oncogene Proteins c-bcl-2 / genetics
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Proto-Oncogene Proteins c-bcl-2 / metabolism*
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RNA, Messenger
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Receptors, Antigen, B-Cell / genetics
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Receptors, Antigen, B-Cell / metabolism
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Receptors, Tumor Necrosis Factor / genetics
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Receptors, Tumor Necrosis Factor / metabolism
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Spleen / cytology*
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T-Lymphocytes / metabolism
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Th1 Cells / metabolism
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Th2 Cells / metabolism
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bcl-X Protein
Substances
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B-Cell Activation Factor Receptor
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BCL2-related protein A1
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Bcl2l1 protein, mouse
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Membrane Proteins
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Minor Histocompatibility Antigens
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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Receptors, Antigen, B-Cell
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Receptors, Tumor Necrosis Factor
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Tnfrsf13c protein, mouse
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bcl-X Protein