Abstract
The expression of cathepsins K, L, B, X and W was studied by quantitative RT-PCR in normal and inflamed gastric mucosa (antrum, corpus, cardia). Cathepsins B, L, K and X were expressed ubiquitously. In contrast, cathepsin W was expressed at very low levels. Infection by Helicobacter pylori caused a significant induction of cathepsin X (p<0.008), whereas the other cathepsins were not or only locally affected by H. pylori infection or reflux disease. Immunohistochemistry revealed specific expression of cathepsin X (macrophages), cathepsin K (parietal cells) and cathepsin W (lymphocytes), whereas cathepsins B and L were predominantly expressed in epithelial cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cathepsin B / genetics
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Cathepsin B / metabolism
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Cathepsin K
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Cathepsin L
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Cathepsin W
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Cathepsins / genetics
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Cathepsins / metabolism*
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Chronic Disease
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Cysteine Endopeptidases / genetics
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Cysteine Endopeptidases / metabolism
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Gastric Mucosa / enzymology*
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Gastric Mucosa / pathology
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Gastritis / enzymology*
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Gastritis / microbiology
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Gastritis / pathology
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Gastroesophageal Reflux / enzymology
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Gene Expression
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Helicobacter Infections / enzymology*
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Helicobacter Infections / pathology
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Humans
Substances
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Cathepsins
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CTSW protein, human
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Cathepsin W
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Cysteine Endopeptidases
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Cathepsin B
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CTSL protein, human
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Cathepsin L
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CTSK protein, human
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Cathepsin K