Abstract
The metabolism of amyloid precursor protein (APP) is central to Alzheimer's disease pathogenesis. Recent data have linked APP and presenilin to the Wnt/wingless signaling pathway. To assess affects of Wnt stimulation on APP isoform expression, we infected PC12 cells and C57MG cells with a retrovirus containing murine Wnt-1. In PC12 cells, Wnt-1 expression is associated with induction of exon 15 deletion from APP mRNA and expression of L-APP. Our data suggest that APP isoform expression is regulated, in part, by the Wnt/wingless signaling pathway.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alternative Splicing / genetics
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Amyloid beta-Protein Precursor / biosynthesis*
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Amyloid beta-Protein Precursor / genetics
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Animals
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Exons / physiology*
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Gene Expression Regulation / physiology
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Mice
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Mice, Inbred C57BL
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PC12 Cells
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Protein Isoforms / biosynthesis
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Protein Isoforms / genetics
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Proto-Oncogene Proteins / biosynthesis*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / physiology*
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Rats
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Retroviridae / genetics
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Sequence Deletion*
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Signal Transduction / genetics
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Signal Transduction / physiology
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Wnt Proteins
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Wnt1 Protein
Substances
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Amyloid beta-Protein Precursor
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Protein Isoforms
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Proto-Oncogene Proteins
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Wnt Proteins
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Wnt1 Protein
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Wnt1 protein, mouse
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Wnt1 protein, rat