Novel techniques for weak alignment of proteins in solution using chemical tags coordinating lanthanide ions

J Biomol NMR. 2004 Jul;29(3):339-49. doi: 10.1023/B:JNMR.0000032611.72827.de.

Abstract

A molecule with an anisotropic magnetic susceptibility is spontaneously aligned in a static magnetic field. Alignment of such a molecule yields residual dipolar couplings and pseudocontact shifts. Lanthanide ions have recently been successfully used to provide an anisotropic magnetic susceptibility in target molecules either by replacing a calcium ion with a lanthanide ion in calcium-binding proteins or by attaching an EDTA derivative to a cysteine residue via a disulfide bond. Here we describe a novel enantiomerically pure EDTA derived tag that aligns stronger due to its shorter linker and does not suffer from stereochemical diversity upon lanthanide complexation. We observed residual (15)N,(1)H-dipolar couplings of up to 8 Hz at 800 MHz induced by a single alignment tensor from this tag.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anisotropy
  • Calcium / chemistry
  • Carbonic Acid / chemistry
  • Disulfides
  • Edetic Acid / chemistry
  • Ions
  • Lanthanoid Series Elements / chemistry*
  • Magnetic Resonance Spectroscopy / methods*
  • Models, Chemical
  • Models, Molecular
  • Models, Statistical
  • Nitrogen
  • Protein Binding
  • Protein Conformation
  • Stereoisomerism

Substances

  • Disulfides
  • Ions
  • Lanthanoid Series Elements
  • Carbonic Acid
  • Edetic Acid
  • Nitrogen
  • Calcium