High-frequency stimulation induces ethanol-sensitive long-term potentiation at glutamatergic synapses in the dorsolateral bed nucleus of the stria terminalis

J Neurosci. 2004 Jun 23;24(25):5741-7. doi: 10.1523/JNEUROSCI.1181-04.2004.

Abstract

Anatomical and functional data support a critical role for the bed nucleus of the stria terminalis (BNST) in the interaction between stress and alcohol/substance abuse. We report here that neurons of the dorsal anterolateral BNST respond to glutamatergic synaptic input in a synchronized way, such that an interpretable extracellular synaptic field potential can be readily measured. High-frequency stimulation of these glutamatergic inputs evoked NMDA receptor (NMDAR)-dependent long-term potentiation (LTP). We found that an early portion of this LTP is reduced by acute exposure to ethanol in a GABA(A) receptor-dependent manner. This effect of ethanol is accompanied by a significant and reversible dose-dependent attenuation of isolated NMDAR signaling and is mimicked by incomplete NMDAR blockade.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium Channels, L-Type / physiology
  • Electric Stimulation
  • Electrophysiology
  • Ethanol / pharmacology*
  • Glutamic Acid / metabolism*
  • In Vitro Techniques
  • Long-Term Potentiation / drug effects
  • Long-Term Potentiation / physiology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Receptors, GABA-A / physiology
  • Receptors, N-Methyl-D-Aspartate / physiology
  • Septal Nuclei / metabolism
  • Septal Nuclei / physiology*
  • Septal Nuclei / ultrastructure
  • Synapses / metabolism
  • Synapses / physiology*

Substances

  • Calcium Channels, L-Type
  • Receptors, GABA-A
  • Receptors, N-Methyl-D-Aspartate
  • Ethanol
  • Glutamic Acid