Abstract
A screening method was established to detect inhibitors of the biosynthetic pathways of aromatic amino acids and para-aminobenzoic acid, the precursor of folic acid, using an agar plate diffusion assay modified as an antagonism test. By this screening method, a family of three novel polycyclic polyketides named as abyssomicins was isolated from a marine strain of Verrucosispora. The main component abyssomicin C inhibits the pathway between chorismate and para-aminobenzoic acid and is strongly active against gram-positive bacteria, including multi-resistant clinical isolates of Staphylococcus aureus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Aminobenzoic Acid / antagonists & inhibitors*
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4-Aminobenzoic Acid / metabolism
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Actinobacteria / chemistry
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Actinobacteria / metabolism*
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Amino Acids, Aromatic / antagonists & inhibitors
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Amino Acids, Aromatic / metabolism
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology
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Chromatography, High Pressure Liquid
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Fermentation
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Geologic Sediments / microbiology
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Macrolides / isolation & purification*
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Macrolides / pharmacology
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Microbial Sensitivity Tests
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Molecular Structure
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Phylogeny
Substances
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Amino Acids, Aromatic
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Anti-Bacterial Agents
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Macrolides
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4-Aminobenzoic Acid