Sequential genotyping within TDT families

Math Med Biol. 2004 Jun;21(2):115-27. doi: 10.1093/imammb/21.2.115.

Abstract

We demonstrate that, given a limited amount of genotyping resources, the power of a TDT study can be increased substantially by genotyping within families sequentially. By sequential genotyping we mean that one parent in a family should be typed and then the decision on whether to continue genotyping the family is based on this parent's genotype. If it is decided to continue genotyping then a further decision is made about whether to genotype the offspring once the second parent has been genotyped. We show that, for a given power, reductions in sample size of over 80% are possible, even for the most robust selection strategies. We discuss the practical application of such sequential genotyping and illustrate its potential using a real data set.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Child
  • Diabetes Mellitus, Type 1 / genetics
  • Female
  • Genetic Diseases, Inborn / genetics*
  • Genotype
  • Humans
  • Linkage Disequilibrium*
  • Male
  • Models, Genetic*
  • Parents