Abstract
The effects of various extracts from oriental medicinal herbs on mast cell-mediated allergic reactions have been investigated. Among the extracts, Arecae semen was the most potent inhibitor of antigen-induced degranulation in RBL-2H3 mast cells. A. semen inhibited DNP-BSA- and compound 48/80-induced degranulation in RBL-2H3 mast cells with IC(50) values of approximately 53 and 52 microg mL(-1), respectively, and inhibited compound 48/80-induced systemic anaphylaxis by 46% at 300 mg kg(-1) in mice. A. semen also inhibited the expression of TNF-alpha and the activation of mitogen activated protein kinase, ERK1/2, which is critical for the production of inflammatory cytokines in mast cells, as indicated by the suppression of the activating phosphorylation of ERK1/2. These results suggest that A. semen may be useful for the treatment of various immediate and delayed allergic diseases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anaphylaxis / chemically induced
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Anaphylaxis / prevention & control*
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Animals
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Anti-Allergic Agents / pharmacology
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Anti-Allergic Agents / therapeutic use*
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Areca*
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Cell Degranulation / drug effects
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Dose-Response Relationship, Drug
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Drugs, Chinese Herbal / pharmacology
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Drugs, Chinese Herbal / therapeutic use
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Fruit
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In Vitro Techniques
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Male
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Mast Cells / drug effects
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Mast Cells / physiology
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Mice
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Mice, Inbred ICR
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Mitogen-Activated Protein Kinase 1 / metabolism
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases / metabolism
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Phosphorylation
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Phytotherapy*
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Tumor Necrosis Factor-alpha / antagonists & inhibitors
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Tumor Necrosis Factor-alpha / biosynthesis
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p-Methoxy-N-methylphenethylamine / pharmacology
Substances
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Anti-Allergic Agents
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Drugs, Chinese Herbal
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Tumor Necrosis Factor-alpha
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p-Methoxy-N-methylphenethylamine
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases