Convergent recognition of the IgE binding site on the high-affinity IgE receptor

Structure. 2004 Jul;12(7):1289-301. doi: 10.1016/j.str.2004.04.015.

Abstract

Two structurally distinct classes of peptides were recently identified by phage display that bind the high-affinity IgE receptor, FcepsilonRI, and block IgE binding and subsequent receptor activation. Both classes adopt highly stable structures in solution, one forming a beta hairpin, with the other forming a helical "zeta" structure. Despite these differences, the two classes bind competitively to the same site on the receptor. Structural analyses of both peptide-receptor complexes by NMR spectroscopy and/or X-ray crystallography reveal that the unrelated peptide scaffolds have nevertheless converged to present a similar three-dimensional surface to interact with FcepsilonRI and that their modes of interaction share a key feature of the IgE-FcepsilonRI complex, the proline/tryptophan sandwich.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Binding, Competitive*
  • Cells, Cultured
  • Crystallography, X-Ray
  • Humans
  • Immunoglobulin E / chemistry
  • Immunoglobulin E / metabolism*
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Peptides / chemistry*
  • Peptides / metabolism
  • Protein Binding
  • Protein Conformation
  • Receptors, IgE / chemistry
  • Receptors, IgE / metabolism*
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism

Substances

  • Peptides
  • Receptors, IgE
  • Recombinant Fusion Proteins
  • Immunoglobulin E

Associated data

  • PDB/1RPQ