Abstract
Objective:
To evaluate the effects of transforming growth factor beta1 (TGFbeta1) autocrine blockage on proliferation activity and drug sensitivity of osteosarcoma. METHODS; Northern blot, MTT determination, and 3H thymidine incorporation were used to investigate the effects of antisense TGF beta1 gene on osteosarcoma.
Results:
The proliferation of osteosarcoma cells transfected by antisense TGF beta1 gene was suppressed markedly, and adriamycin sensitivity was significantly increased.
Conclusion:
Blockage of osteosarcoma cells TGF beta1 autocrine loop inhibits cell proliferation and enhances chemotherapy sensitivity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / pharmacology
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Antisense Elements (Genetics) / biosynthesis
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Antisense Elements (Genetics) / genetics
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Autocrine Communication / drug effects*
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Bone Neoplasms / metabolism
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Bone Neoplasms / pathology*
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Cell Division / drug effects
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Cell Line, Tumor
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Doxorubicin / pharmacology
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Humans
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Osteosarcoma / metabolism
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Osteosarcoma / pathology*
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RNA, Messenger / genetics
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Transfection
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Transforming Growth Factor beta / biosynthesis
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Transforming Growth Factor beta / genetics*
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Transforming Growth Factor beta1
Substances
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Antineoplastic Agents
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Antisense Elements (Genetics)
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RNA, Messenger
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TGFB1 protein, human
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Transforming Growth Factor beta
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Transforming Growth Factor beta1
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Doxorubicin