Abstract
Three floR genes were cloned from calf pathogenic Escherichia coli strains, and the efflux-mediated accumulation of florfenicol in the floR gene-JM109 E. coli system was determined by HPLC. The floR genes resulted in a 1356-bp fragment covering the ORF in region 66-1280 coding for 404 amino acids. The common motifs of 12-transmembrane segments efflux pumps family were conserved in the deduced floR amino acid sequences. HPLC results indicated a significant difference in florfenicol accumulation between florfenicol-resistant strains and the susceptible strains, which was almost reversed by the addition of a proton motive force blocker. These results suggest that the florfenicol resistance mediated by the floR gene involves active efflux of florfenicol.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Anti-Bacterial Agents / metabolism
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Anti-Bacterial Agents / pharmacology*
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Biological Transport, Active
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Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
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Cattle
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Cattle Diseases / microbiology
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Cloning, Molecular
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Conserved Sequence
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification
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Diarrhea / microbiology
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Diarrhea / veterinary
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Drug Resistance, Bacterial*
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Escherichia coli / drug effects*
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Escherichia coli / genetics*
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Escherichia coli / isolation & purification
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Escherichia coli / metabolism
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / veterinary
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Genes, Bacterial
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Molecular Sequence Data
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Open Reading Frames
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Phylogeny
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Protein Sorting Signals
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Proton Pump Inhibitors
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Proton-Motive Force / drug effects
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Sequence Analysis, DNA
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Thiamphenicol / analogs & derivatives*
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Thiamphenicol / metabolism
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Thiamphenicol / pharmacology*
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Uncoupling Agents / pharmacology
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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Protein Sorting Signals
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Proton Pump Inhibitors
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Uncoupling Agents
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Carbonyl Cyanide m-Chlorophenyl Hydrazone
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florfenicol
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Thiamphenicol
Associated data
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GENBANK/AY499129
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GENBANK/AY499130
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GENBANK/AY517519