Fluorometric assay for determining epoxide hydrolase activity

Biotechnol Lett. 2004 May;26(10):835-9. doi: 10.1023/b:bile.0000025887.36874.33.

Abstract

We have developed a rapid screening procedure that enables the screening of hundreds of enzyme samples or variants for epoxide hydrolase activity towards any substrate. The procedure detects the products of the enzymatic reaction via periodate cleavage and remaining fluorescence of carboxyfluorescein.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Enzyme Activation
  • Epoxide Hydrolases / analysis*
  • Epoxide Hydrolases / chemistry*
  • Fluoresceins*
  • Fluorometry / methods*
  • Streptomyces antibioticus / enzymology*

Substances

  • Fluoresceins
  • 6-carboxyfluorescein
  • Epoxide Hydrolases