Abstract
The apical membrane antigen 1 of Plasmodium falciparum is one of the leading candidate antigens being developed as a vaccine to prevent malaria. This merozoite transmembrane protein has an ectodomain that can be divided into three subdomains (D I, D II, and D III). We have previously expressed a major portion of this ectodomain and have shown that it can induce antibodies that prevent merozoite invasion into red blood cells in an in vitro growth and invasion assay. To analyze the antibody responses directed against the individual subdomains, we constructed six different genes that express each of the domains separately (D I, D II, or D III) or in combination with another domain (D I+II, D II+III, or D I+III). These proteins were purified and used to immunize rabbits to raise construct-specific antibodies. We demonstrated that D I+II induced a significant amount of the growth-inhibitory antibodies active in the growth and invasion assay.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antibodies, Protozoan / blood*
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Antibodies, Protozoan / immunology
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Antigens, Protozoan / chemistry*
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Antigens, Protozoan / genetics
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Antigens, Protozoan / immunology*
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Antigens, Protozoan / metabolism
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Erythrocytes / parasitology
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Immunization
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Malaria Vaccines / administration & dosage
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Malaria Vaccines / immunology
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Malaria, Falciparum / prevention & control
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Membrane Proteins / chemistry*
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Membrane Proteins / genetics
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Membrane Proteins / immunology*
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Membrane Proteins / metabolism
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Plasmodium falciparum / chemistry
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Plasmodium falciparum / growth & development
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Plasmodium falciparum / immunology*
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Plasmodium falciparum / pathogenicity*
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Protozoan Proteins / chemistry*
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Protozoan Proteins / genetics
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Protozoan Proteins / immunology*
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Protozoan Proteins / metabolism
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Rabbits
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Recombinant Proteins / metabolism
Substances
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Antibodies, Protozoan
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Antigens, Protozoan
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Malaria Vaccines
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Membrane Proteins
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Protozoan Proteins
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Recombinant Proteins
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apical membrane antigen I, Plasmodium