Abstract
N19, a string of human universal CD4 T-cell epitopes from various pathogen-derived antigens, was shown to exert a stronger carrier effect than CRM197 for the induction of anti-group C Neisseria meningitidis capsular polysaccharide (MenC), after immunization of mice with various dosages of N19-MenC or CRM-MenC conjugate vaccines. After two immunizations, the N19-based construct induced anti-MenC antibody and protective bactericidal antibody titers higher than those induced by three doses of the CRM-MenC conjugate and required lower amounts of conjugate. N19-based conjugates are superior to CRM-based conjugates to induce protective immune responses to MenC conjugates.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Bacterial / blood
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Bacterial Proteins / immunology
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CD4-Positive T-Lymphocytes / immunology*
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Diphtheria Toxin / immunology
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Drug Carriers
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Epitopes, T-Lymphocyte / chemistry
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Epitopes, T-Lymphocyte / genetics
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Epitopes, T-Lymphocyte / immunology*
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Epitopes, T-Lymphocyte / metabolism
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Female
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Humans
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Meningitis, Meningococcal / prevention & control
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Meningococcal Vaccines / administration & dosage
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Meningococcal Vaccines / immunology*
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Mice
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Molecular Sequence Data
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Neisseria meningitidis, Serogroup C / immunology*
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Recombinant Proteins / administration & dosage
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Recombinant Proteins / immunology*
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Vaccines, Conjugate / administration & dosage
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Vaccines, Conjugate / immunology*
Substances
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Antibodies, Bacterial
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Bacterial Proteins
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Diphtheria Toxin
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Drug Carriers
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Epitopes, T-Lymphocyte
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Meningococcal Vaccines
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Recombinant Proteins
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Vaccines, Conjugate
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CRM197 (non-toxic variant of diphtheria toxin)