Abstract
Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release (FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEARnetwork promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anaphase
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cell Nucleolus / metabolism*
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Cyclin B / metabolism
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Cyclin B1
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Cyclin-Dependent Kinases / metabolism*
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DNA, Ribosomal / metabolism
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Meiosis
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Metaphase
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Mitosis*
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Mutation
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Phosphorylation
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Protein Kinases / metabolism
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Protein Serine-Threonine Kinases
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Protein Tyrosine Phosphatases / metabolism*
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
Substances
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CDC14 protein, S cerevisiae
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Cell Cycle Proteins
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Cyclin B
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Cyclin B1
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DNA, Ribosomal
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Net1 protein, S cerevisiae
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Nuclear Proteins
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins
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Protein Kinases
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Protein Serine-Threonine Kinases
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CDC5 protein, S cerevisiae
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Cyclin-Dependent Kinases
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Protein Tyrosine Phosphatases