Experimental acute traumatic injury of the adult rat spinal cord by a subdural inflatable balloon: methodology, behavioral analysis, and histopathology

J Neurosci Res. 1992 Aug;32(4):539-50. doi: 10.1002/jnr.490320409.

Abstract

We describe an experimental model to produce closed traumatic injuries to the spinal cord of adult rats. This model uses an inflatable balloon that is introduced in the dorsal subdural space and moved to a location rostral to the laminectomy site. The spinal cord trauma can be graded by varying either the duration of compression or the volume of saline used to inflate the balloon. The locomotor deficit of animals with various degrees of injury has been assessed at increasing delays after trauma. The parameters generating transient or definitive deficits of varying intensity were defined. Some injured animals underwent nuclear magnetic resonance imaging. Detailed histopathological studies demonstrated that the extent of the spinal lesion was significantly correlated with the physical parameters of compression and with the severity of the behavioral deficit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal / physiology*
  • Disease Models, Animal
  • Histocytochemistry
  • Magnetic Resonance Imaging
  • Male
  • Psychomotor Performance
  • Rats
  • Rats, Inbred Strains
  • Spinal Cord / pathology
  • Spinal Cord Injuries / pathology*
  • Spinal Cord Injuries / psychology