Abstract
Transposable element P of Drosophila melanogaster is one of the best-characterized eukaryotic transposons. Successful transposition requires the interaction between transposase complexes at both termini of the P element. Here we found that insertion of one or two copies of the Su(Hw) insulator in the P transposon reduces the frequency of its transposition. Inactivation of a Mod(mdg4) component of the Su(Hw) insulator suppresses the insulator effect. Thus, the Su(Hw) insulator can modulate interactions between transposase complexes bound to the ends of the P transposon in germ cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Crosses, Genetic
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DNA Transposable Elements / genetics*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Drosophila Proteins
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Drosophila melanogaster / genetics*
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism*
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Repressor Proteins
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transformation, Genetic
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Transposases / metabolism*
Substances
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DNA Transposable Elements
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DNA-Binding Proteins
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Drosophila Proteins
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Nuclear Proteins
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Recombinant Proteins
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Repressor Proteins
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Transcription Factors
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mdg4 protein (gypsy)
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su(Hw) protein, Drosophila
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Transposases