Abstract
The mechanisms by which changes in glucose concentration regulate gene expression and insulin secretion in pancreatic islet beta-cells are only partly understood. Here we describe the development of new technologies for examining these processes at the level of single living beta-cells. We also present recent findings, made using these and other techniques, which implicate a role for adenosine 5'-monophosphate-activated protein kinase in glucose signaling in these cells.
Publication types
-
Research Support, Non-U.S. Gov't
-
Review
MeSH terms
-
AMP-Activated Protein Kinases / metabolism*
-
Animals
-
Gene Expression Profiling*
-
Gene Expression Regulation
-
Glucose / metabolism*
-
Green Fluorescent Proteins / metabolism
-
Humans
-
Insulin / metabolism*
-
Insulin Secretion
-
Insulin-Secreting Cells / cytology*
-
Insulin-Secreting Cells / metabolism
-
Mice
-
Microscopy, Fluorescence / methods
-
Models, Biological
-
Phosphatidylinositol 3-Kinases / metabolism
-
Signal Transduction
-
Time Factors
Substances
-
Insulin
-
Green Fluorescent Proteins
-
Phosphatidylinositol 3-Kinases
-
AMP-Activated Protein Kinases
-
Glucose