Abstract
We report a rapid and accurate real-time PCR-based method to quantify wild-type and lamivudine-resistant hepatitis B virus by using a common forward primer paired with different reverse primers. Excellent concordance was demonstrated between sequencing and the discriminatory real-time assay; however, a mixture of quasispecies was more frequently detected by discriminatory real-time PCR.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Computer Systems
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DNA Primers
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Genetic Variation
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Hepatitis B / diagnosis*
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Hepatitis B / drug therapy
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Hepatitis B virus / classification
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Hepatitis B virus / drug effects
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Hepatitis B virus / genetics*
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Hepatitis B virus / isolation & purification
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Humans
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Lamivudine / pharmacology*
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Longitudinal Studies
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Polymerase Chain Reaction / methods
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Reverse Transcriptase Inhibitors / pharmacology
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Viral Load
Substances
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DNA Primers
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Reverse Transcriptase Inhibitors
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Lamivudine